The gender discrimination of yellow-headed temple turtles is mostly based on the morphological differences of their tails and plastrons. Under normal circumstances, the tails of female turtles are shorter and thinner; the tails of male turtles are longer and thicker, and this feature will become more obvious as they grow up.

Another way is to observe the shape of their plastrons, female turtles The plastron is relatively flat, and the distance between the rear edge of the plastron and the anus is very close; the center of the plastron of the male turtle is concave, and the distance between the rear edge of the plastron and the anus is relatively far.