The male and female eastern box turtles are easier to distinguish. The males have thicker tails and the females are smaller. In addition to judging by the tail, it can also be identified by the body size and eyes of the eastern box turtle.

The males of eastern box turtles have longer nails, and the back is longer than the front Broad, plastron sunken, red irises of the eyes. The female eastern box turtle has a broad carapace, a flat plastron without depression, and a yellowish-brown iris of the eyes.