The methods of identifying male and female Mesoamerican river turtles are as follows:

From body size Look: Male Mesoamerican river turtles are smaller, whereas female Mesoamerican river turtles are larger.

From the tail: Male Mesoamerican river turtles have longer tails, whereas female Mesoamerican river turtles have shorter tails.

From the point of view of the plastron: the center of the plastron of the male Mesoamerican river turtle is slightly concave, whereas the center of the plastron of the female Mesoamerican river turtle is flat.

And male Mesoamerican river turtles have hooked spines at the ends, and two rows of denser patchwork junctions on the medial surface of the hind legs.