Judging by appearance, male Florida red-bellied tortoises have a hollow plastron. The head will be relatively smaller than the female Florida red-bellied tortoise, the color of the shell will be darker, the torso will be longer and thinner, and the tail will be long and thin. The female Florida red-bellied tortoise will have a flat plastron and a shallow shell. , the body is short and thick.

Male Florida red-bellied tortoise has a peculiar smell and is stronger, female Florida red-bellied tortoise The odor of belly turtles is relatively mild. Judging from the cloaca, the cloaca of the male Florida red-bellied turtle is closer to the tail and the hole shape is longer, while the cloaca of the female Florida red-bellied turtle is closer to the plastron, and the hole shape will be relatively round.